UV-Vis or ultraviolet-visible spectrometer (spectrophotometer)

اسپکتروفتومتر

UV/VIS Spectrometry

A UV-Vis spectrophotometer is a very common tool in a chemist's toolbox. This versatile measurement technique has a secure place in the chemistry laboratory.

A spectrophotometer, also known as a UV-Vis spectrometer, is a device for measuring the amount of a substance based on its electromagnetic absorption. Most of the substances analyzed in the spectrometer are in liquid form. Light is emitted in a visible spectrophotometer using a tungsten lamp, and in a UV spectrometer using a deuterium lamp. A spectrophotometer is used for the qualitative and quantitative measurement of a wide range of substances in various scientific and research fields such as chemistry, biochemistry, pharmacy, materials and the environment.

 

How a UV-Vis Spectrophotometer Works

UV-Vis spectroscopy (or spectrophotometry) is a quantitative method used to measure the amount of light absorbed by a chemical. This is done by measuring the intensity of light transmitted through a sample (relative to the intensity of light transmitted through a blank sample). This method can be used for a variety of sample types, including liquids, solids, thin films, and glass.

Spectrophotometry is the quantitative measurement of the absorption/transmission or reflectance of a substance as a function of wavelength. Although it is called UV-Vis, the wavelength range typically used ranges from 190 nm to 1100 nm in the near infrared.

Using a spectrophotometer and making absorption/transmission measurements, we can determine the amount (or concentration) of a known chemical by studying the number of photons (light intensity) that reach the detector.

The greater the absorption of light by a substance at a specific wavelength, the greater the concentration of the known substance.

 

Applications of UV-Vis Spectrophotometer

UV/Vis spectroscopy is used in analytical chemistry for the quantitative determination of various analytes, including the transport of metal ions, organic compounds, and biological macromolecules.

UV-Vis spectrophotometer analysis

# Specialized analysis of UV-Vis spectrophotometer or ultraviolet visible spectrophotometer Abrizan Company - manufacturer of scale-removing, anti-scaling, and anti-corrosion materials

author: تیم تولیدمحتوای آبریزان

share :

Submit your opinion

Your email address will not be published.


Related Articles

کروماتوگرافی گازی و طیف سنجی جرمی
25/12/2024

Gas chromatography/mass spectrometry (GC/MS)

Gas chromatography-mass spectrometry separates chemical mixtures (gas phase) and identifies components at the molecular level (mass phase). It is one of the most accurate tools for analyzing environmental samples.

نورسنجی شعله
25/12/2024

Flame Photometer

Flame photometry is used to determine the concentration of alkaline earth and alkaline elements (in inorganic chemical analysis to determine the concentration of certain metal ions including sodium, potassium, lithium, and calcium) in aqueous solutions.

طیف سنج مادون قرمز
24/12/2024

FTIR Infrared Spectrometer

Fourier transform infrared (FTIR) spectroscopy is a method that scientists use to determine the structure of molecules by absorbing characteristic infrared radiation. The infrared spectrum is the vibrational spectrum of a molecule. When exposed to infrared radiation, sample molecules selectively absorb radiation of specific wavelengths, which causes a change in the dipole moment of the sample molecules.

دستگاه اتولب
24/12/2024

Autolab device

The Autolab is an ideal choice for those looking for a low-cost, compact, yet high-performance electrochemical analyzer. Fully integrated with NOVA software, this compact instrument can perform resistance measurements in the microhertz to megahertz frequency range.

کروماتوگرافی مایع با کارایی بالا
24/12/2024

High-performance liquid chromatography (HPLC)

HPLC analysis, or high-performance liquid chromatography, is suitable for analyzing and separating chemical and plant compounds and is used in various fields of chemistry, pharmacy, industrial chemistry, and clinical diagnostics.

غشاهای شیشه ای
23/12/2024

Glass membranes

A silicate glass used for glass membranes consists of an infinite three-dimensional structure of four negatively charged SiO4 groups, in which each silicon is bonded to four oxygens and each oxygen to two silicons. Within the network of this structure there are enough cations to neutralize the negative charge of the silicate group. Singly charged cations such as sodium and lithium move in the network and are responsible for electrical conduction within the membrane.

pH متری و روش های آن
23/12/2024

pH measurement and its methods

The pH of water determines the concentration of hydrogen ions and its acidity or alkalinity. Pure water dissociates to a very small extent into H and OH ions. The pH of drinking water should usually be around 7 (neutral). If the pH is less than 7, the water is acidic, and if the pH is above 7, the water is alkaline.

لوزی خطر
23/12/2024

Danger rhombus

The National Fire Protection Association (NFPA) has developed a standard called NFPA 704 that is used to identify hazards from materials (especially chemicals). Among occupational health and safety experts, this standard has become known as the Hazard Diamond. It seems that the Rescue Diamond might have been a more appropriate name for the Hazard Diamond!